Genomics Core Facility

Function

Welcome to the Genomics Core Facility (GCF), located in the Department of Biology at East Carolina University (ECU). We provide rapid, dependable and economical service for Sanger and next-generation sequencing to the research community of ECU and researchers outside ECU.

Instrumentation

Sanger sequencing utilizes the SeqStudio Genetic Analyzer from Applied Biosystems. This fluorescent dye-labeled genetic analysis system uses a four-capillary array which enables both sequencing and fragment analysis applications.

To determine the quality of RNA and DNA samples, we use the Agilent Bioanalyzer. The Bioanalyzer only requires 1ul of sample and is sensitive enough to detect pico amounts of RNA, and the RIN (RNA Integrity Number), developed by Agilent, is the gold standard for RNA quality.

RNA, DNA, or protein quantification is determined using the Qubit 4 Fluorometer.   This instrument accurately and quickly measures the concentration of DNA, RNA, or protein.

Coming soon: Illumina Miniseq

The MiniSeq System delivers the power and confidence of proven Illumina next-generation sequencing (NGS) technology in an accessible sequencing solution. It features cost-efficient sequencing, even for low numbers of samples. Its streamlined workflow enables rapid sequencing of both DNA and RNA. The MiniSeq System integrates clonal amplification, sequencing by synthesis, and base calling into a single benchtop instrument.

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